We thank the LMU Innovative Initiative Functional NanoSystem (FUNS) and the Excellence Cluster NanoSystems Initiative Munich (NIM) for financial support.
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Communication
Optical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding†
Article first published online: 23 FEB 2010
DOI: 10.1002/anie.200903998
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Baaske, P., Wienken, Christoph J., Reineck, P., Duhr, S. and Braun, D. (2010), Optical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding. Angew. Chem. Int. Ed., 49: 2238–2241. doi: 10.1002/anie.200903998
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Publication History
- Issue published online: 10 MAR 2010
- Article first published online: 23 FEB 2010
- Manuscript Received: 20 JUL 2009
Funded by
- LMU Innovative Initiative Functional NanoSystem (FUNS)
- Excellence Cluster NanoSystems Initiative Munich (NIM)
Keywords:
- aptamers;
- DNA;
- drug discovery;
- immobilization-free methods;
- proteins
Some like it hot: A robust and fast method for characterizing aptamers relies on the distinct thermophoretic movements of molecules in microscopic temperature gradients (see diagram). The binding properties of proteins and even small molecules can be measured within seconds, and less than 1 μL of sample is required. Notably, the technique works well in complex liquids such as human serum.