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Keywords:

  • native chemical ligation;
  • protein engineering;
  • protein modifications;
  • pyrrolysine;
  • ubiquitination
Thumbnail image of graphical abstract

The art of stitching proteins: D-Cys-ε-Lys and its diastereomer L-Cys-ε-Lys read through the UAG codon (see scheme). As the resulting proteins can participate in native chemical ligation (NCL), this process provides a means to prepare proteins chemoselectively modified (e.g. ubiquitinated) through a peptidic side chain located at the ε position of a rationally selected lysine residue.