BTB-1: A Small Molecule Inhibitor of the Mitotic Motor Protein Kif18A


  • We gratefully acknowledge funding by the DAAD and Marie Curie Actions 6. We also thank Prof. Dr. W. Hofer and the members of the Mayer lab for helpful discussions.


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Turning the motor off: A malachite green based assay leads to the identification of BTB-1 (see picture), the first small-molecule inhibitor of the mitotic motor protein Kif18A. BTB-1 reversibly inhibits the ATPase activity of the recombinant motor domain of Kif18A in vitro (see picture; red microtubules, blue/black structure =Kif18A) and will be a valuable tool to dissect the mechanochemical properties of Kif18A.