Advertisement

BTB-1: A Small Molecule Inhibitor of the Mitotic Motor Protein Kif18A

Authors


  • We gratefully acknowledge funding by the DAAD and Marie Curie Actions 6. We also thank Prof. Dr. W. Hofer and the members of the Mayer lab for helpful discussions.

Abstract

original image

Turning the motor off: A malachite green based assay leads to the identification of BTB-1 (see picture), the first small-molecule inhibitor of the mitotic motor protein Kif18A. BTB-1 reversibly inhibits the ATPase activity of the recombinant motor domain of Kif18A in vitro (see picture; red microtubules, blue/black structure =Kif18A) and will be a valuable tool to dissect the mechanochemical properties of Kif18A.

Ancillary