These authors contributed equally to this work.
Single-Molecule DNA Biosensors for Protein and Ligand Detection†
Article first published online: 13 JAN 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 7, pages 1316–1320, February 8, 2010
How to Cite
Lymperopoulos, K., Crawford, R., Torella, Joseph P., Heilemann, M., Hwang, L., Holden, Seamus J. and Kapanidis, Achillefs N. (2010), Single-Molecule DNA Biosensors for Protein and Ligand Detection. Angew. Chem. Int. Ed., 49: 1316–1320. doi: 10.1002/anie.200904597
We thank Dr. M. Brenowitz (Albert Einstein College of Medicine) and Dr. R. Ebright (HHMI/Rutgers University) for plasmids, Dr. S. Weiss (UCLA) for software, and L. Sattary and J. Ghadiali for assistance. Funding was provided by the UK Bionanotechnology IRC, the EU (MIRG-CT-2005-031079), EPSRC (EP/D058775), and the Wellcome Trust (VS/06/OX/A4). M.H. was supported by a DAAD fellowship.
- Issue published online: 1 FEB 2010
- Article first published online: 13 JAN 2010
- Manuscript Revised: 3 NOV 2009
- Manuscript Received: 18 AUG 2009
- UK Bionanotechnology IRC
- EU. Grant Number: MIRG-CT-2005-031079
- EPSRC. Grant Number: EP/D058775
- Wellcome Trust. Grant Number: VS/06/OX/A4
- laser spectroscopy;
- protein–DNA interactions;
- single-molecule studies;
- transcription factors
Transcription factors (TFs) control gene expression and are promising biomarkers for diseases. In a new, robust, and sensitive single-molecule fluorescence assay TFs and associated small molecules (such as nucleotides and sugars) are detected based on DNA coincidence of the two halves of the binding site (see picture; S=stoichiometry). The assay is compatible with measurements in solution, on solid supports, and even in complex biological samples.