Synthesis of Fe3O4@SiO2@PMMA Core–Shell–Shell Magnetic Microspheres for Highly Efficient Enrichment of Peptides and Proteins for MALDI-ToF MS Analysis


  • This work was supported by the National Natural Science Foundation of China (Project: 20875017), the Technological Innovation Program of Shanghai (09JC1401100), the National Basic Research Priorities Program (Project: 2007CB914100/3), and the Shanghai Leading Academic Discipline Project (B109).


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Particularly enriching: Core–shell–shell Fe3O4§SiO2§PMMA composite microspheres (see picture; PMMA=poly(methyl methacrylate)) were synthesized readily by combining a sol-gel approach with a seeded aqueous-phase radical polymerization method. The microspheres were successfully applied toward the effective, rapid, and convenient enrichment of low-concentration peptides and proteins for mass spectrometric analysis.