Direct Visualization of Disulfide Bonds through Diselenide Proxies Using 77Se NMR Spectroscopy


  • This work was supported by Discovery Grants DP0774245 and DP0878450 from the Australian Research Council and the Queensland Smart State Research Facilities Fund.


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Se-ing is believing: Many proteins are cross-braced by disulfide bonds that frequently play key roles in protein structure, folding, and function. Unfortunately, the methods available for assignment of disulfide-bond connectivities in proteins are technically difficult and prone to misinterpretation. Now disulfide bond connectivities in native proteins can be visualized directly using 77Se NMR spectroscopy.