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Keywords:

  • disulfide bonds;
  • NMR spectroscopy;
  • peptides;
  • selenium;
  • solid-phase synthesis
Thumbnail image of graphical abstract

Se-ing is believing: Many proteins are cross-braced by disulfide bonds that frequently play key roles in protein structure, folding, and function. Unfortunately, the methods available for assignment of disulfide-bond connectivities in proteins are technically difficult and prone to misinterpretation. Now disulfide bond connectivities in native proteins can be visualized directly using 77Se NMR spectroscopy.