Fluorescence Turn-On Detection of a Protein through the Reduced Aggregation of a Perylene Probe

Authors

  • Bin Wang,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (China), Fax: (+86) 431-8526-2710
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  • Cong Yu Prof. Dr.

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (China), Fax: (+86) 431-8526-2710
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  • This research was supported by the “100 Talents” program (initiation support) of the Chinese Academy of Sciences and the National Natural Science Foundation of China (No. 20845006).

Abstract

original image

Sensitivity that won't break the bank: The addition of an anti-lysozyme DNA aptamer (a polyanion) to a cationic perylene probe that exists in aqueous solution as a mixture of the fluorescent free monomer and aggregate forms shifted the equilibrium and resulted in fluorescence quenching (see schematic representation). The subsequent introduction of lysozyme weakened DNA binding to the perylene aggregates and led to fluorescence recovery.

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