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Efficient Delivery of Bioactive Antibodies into the Cytoplasm of Living Cells by Charge-Conversional Polyion Complex Micelles

Authors

  • Yan Lee Dr.,

    1. Department of Chemistry, Seoul National University, Sillim-dong, Gwanak-gu, Seoul 151-742 (Korea)
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  • Takehiko Ishii Dr.,

    1. Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan), Fax: (+81) 3-5841-7139
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  • Hyun Jin Kim,

    1. Department of Materials Engineering, The University of Tokyo (Japan)
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  • Nobuhiro Nishiyama Dr.,

    1. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo (Japan)
    2. Center for Nanobio Integration, The University of Tokyo (Japan)
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  • Yoshiyuki Hayakawa,

    1. Department of Materials Engineering, The University of Tokyo (Japan)
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  • Keiji Itaka Dr.,

    1. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo (Japan)
    2. Center for Nanobio Integration, The University of Tokyo (Japan)
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  • Kazunori Kataoka Prof. Dr.

    1. Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan), Fax: (+81) 3-5841-7139
    2. Department of Materials Engineering, The University of Tokyo (Japan)
    3. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo (Japan)
    4. Center for Nanobio Integration, The University of Tokyo (Japan)
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  • This work was supported by a Core Research for Evolutional Science and Technology (CREST) grant from the Japan Science and Technology Agency (JST).

Abstract

original image

Stand and deliver! Immunoglobulin G (IgG) can be delivered into the cytoplasm of living cells by charge-conversional modification followed by treatment with a cationic block copolymer to form polyion complex (PIC) micelles (see picture). The bioactivity of the IgG selectively recovers in the cell in a pH-dependent manner, thereby controlling the growth of human hepatoma cells through IgG binding to intracellular target molecules.

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