This work was supported by grants from the NIH, the MRC, and the European Commission’s seventh Framework Programme (FP7) READNA Consortium. D.S. was supported by a BBSRC Doctoral Training Grant.
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Communication
Multiple Base-Recognition Sites in a Biological Nanopore: Two Heads are Better than One†
Article first published online: 11 DEC 2009
DOI: 10.1002/anie.200905483
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Stoddart, D., Maglia, G., Mikhailova, E., Heron, A. and Bayley, H. (2010), Multiple Base-Recognition Sites in a Biological Nanopore: Two Heads are Better than One. Angewandte Chemie International Edition, 49: 556–559. doi: 10.1002/anie.200905483
- †
Publication History
- Issue published online: 7 JAN 2010
- Article first published online: 11 DEC 2009
- Manuscript Received: 30 SEP 2009
Funded by
- NIH
- MRC
- European Commission’s seventh Framework Programme (FP7) READNA Consortium
- BBSRC
Keywords:
- α-hemolysin;
- DNA sequencing;
- nanopores;
- protein engineering;
- single-nucleotide analysis
Graphical Abstract
The α-hemolysin nanopore is a promising sensor for ultra-rapid sequencing of DNA strands within nanopores. By using immobilized synthetic oligonucleotides, it is shown that additional sequence information can be gained when two recognition sites, rather than one, are employed within a single nanopore (see picture).