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Structures of DNA Polymerases Caught Processing Size-Augmented Nucleotide Probes

Authors

  • Karin Betz,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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    • These authors contributed equally.

  • Frank Streckenbach,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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    • These authors contributed equally.

  • Andreas Schnur Dr.,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • Thomas Exner Prof. Dr.,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • Wolfram Welte Prof. Dr.,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • Kay Diederichs Prof. Dr.,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • Andreas Marx Prof. Dr.

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany), Fax: (+49) 7531-88-5140
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  • We gratefully acknowledge funding by the DFG.

Abstract

original image

The right fit: Synthetic nucleotide analogues are widely used to investigate the mechanisms that govern DNA polymerase selectivity—processes that are crucial for the survival of every living organism. The first crystal structures of size-augmented 4′-methylated and 4′-ethylated thymidine triphosphates (TTPs) in complex with a DNA polymerase have been elucidated (picture: superposition of three DNA polymerase structures in complex with TTPs).

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