Inside Cover

Inside Cover: Design and Synthesis of a Homogeneous Erythropoietin Analogue with Two Human Complex-Type Sialyloligosaccharides: Combined Use of Chemical and Bacterial Protein Expression Methods (Angew. Chem. Int. Ed. 50/2009)

  1. Kiriko Hirano1,
  2. Derek Macmillan Prof. Dr.2,
  3. Katsunari Tezuka Dr.3,
  4. Takashi Tsuji Prof. Dr.4,
  5. Yasuhiro Kajihara Prof. Dr.1,5

Article first published online: 11 NOV 2009

DOI: 10.1002/anie.200905761

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 48, Issue 50, page 9378, December 7, 2009

Additional Information

How to Cite

Hirano, K., Macmillan, D., Tezuka, K., Tsuji, T. and Kajihara, Y. (2009), Inside Cover: Design and Synthesis of a Homogeneous Erythropoietin Analogue with Two Human Complex-Type Sialyloligosaccharides: Combined Use of Chemical and Bacterial Protein Expression Methods (Angew. Chem. Int. Ed. 50/2009). Angew. Chem. Int. Ed., 48: 9378. doi: 10.1002/anie.200905761

Author Information

  1. 1

    International Graduate School of Arts and Sciences, Yokohama City University, 22-2, Seto, Kanazawa-ku, Yokohama, 236-0027 (Japan)

  2. 2

    Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ (UK)

  3. 3

    Research Project for Glycoprotein Engineering funded by Otsuka Chemical Co., Ltd., Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510 (Japan)

  4. 4

    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510 (Japan)

  5. 5

    Current address: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043 (Japan)

Publication History

  1. Issue published online: 30 NOV 2009
  2. Article first published online: 11 NOV 2009

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Keywords:

  • erythropoietin;
  • glycopeptides;
  • glycoproteins;
  • native chemical ligation;
  • protein expression
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Bioengineering of proteins using mammalian cells has facilitated the development of potential pharmaceutical erythropoietins (EPOs) that bear N-linked oligosaccharides. In their Communication on page 9557 ff., Y. Kajihara and co-workers report the chemical synthesis of a homogeneous bioactive EPO analogue, which was prepared by native chemical ligation between a synthetic glycopeptide-α-thioester with two complex-type sialyloligosaccharides and a large polypeptide chain, obtained by expression in E. coli.

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