Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules


  • We thank M. Gautel, his colleagues A. Alexandrovich and B. Brandmeier for the titin kinase protein constructs, and S. Stahl, A. Fornof, and H. Engelke for helpful diskussions. E.M.P. thanks the international Doktorate Program NanoBioTechnology (IDK-NTB) for support. This work was funded by the Deutsche Forschungsgemeinschaft.


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Feel the force: A new AFM-based single-molecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.