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Keywords:

  • enzymes;
  • force spectroscopy;
  • scanning probe microscopy;
  • sensors;
  • single-molecule studies
Thumbnail image of graphical abstract

Feel the force: A new AFM-based single-molecule pump-and-probe protocol has been used to investigate the function of different force-induced conformations of proteins (see picture). It was found that the autoinhibited enzyme titin kinase reaches its ability to bind ATP after the first two barriers of the complex mechanical activation pathway.