We thank Dr. Justin Ragains for helpful advice and Alex Chudnovsky for assistance with tissue culture. This research was supported in part by NIH grants U01-HL080731, T32-CA79443, P50-CA86355, and RO1-EB010011.
Bioorthogonal Turn-On Probes for Imaging Small Molecules inside Living Cells†
Article first published online: 19 MAR 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 16, pages 2869–2872, April 6, 2010
How to Cite
Devaraj, Neal K., Hilderbrand, S., Upadhyay, R., Mazitschek, R. and Weissleder, R. (2010), Bioorthogonal Turn-On Probes for Imaging Small Molecules inside Living Cells. Angew. Chem. Int. Ed., 49: 2869–2872. doi: 10.1002/anie.200906120
- Issue published online: 30 MAR 2010
- Article first published online: 19 MAR 2010
- Manuscript Revised: 20 JAN 2010
- Manuscript Received: 30 OCT 2009
- NIH. Grant Numbers: U01-HL080731, T32-CA79443, P50-CA86355, RO1-EB010011
- bioorthogonal reactions;
- fluorogenic tags;
Glowing tags: A series of activatable (“turn-on”) tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity (see picture). The novel turn-on probes were applied for intracellular live-cell imaging of a microtubuli-binding trans-cyclooctene modified taxol.