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Keywords:

  • bioorthogonal reactions;
  • cycloaddition;
  • fluorogenic tags;
  • imaging;
  • tetrazine
Thumbnail image of graphical abstract

Glowing tags: A series of activatable (“turn-on”) tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity (see picture). The novel turn-on probes were applied for intracellular live-cell imaging of a microtubuli-binding trans-cyclooctene modified taxol.