Communication

An Efficient Protocol for NMR-Spectroscopy-Based Structure Determination of Protein Complexes in Solution†

  1. Bernd Simon Dr.3,
  2. Tobias Madl Dr.1,2,
  3. Cameron D. Mackereth Dr.4,
  4. Michael Nilges Prof. Dr.5,
  5. Michael Sattler Prof. Dr.1,2

Article first published online: 10 FEB 2010

DOI: 10.1002/anie.200906147

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 49, Issue 11, pages 1967–1970, March 8, 2010

Additional Information

How to Cite

Simon, B., Madl, T., Mackereth, C., Nilges, M. and Sattler, M. (2010), An Efficient Protocol for NMR-Spectroscopy-Based Structure Determination of Protein Complexes in Solution. Angewandte Chemie International Edition, 49: 1967–1970. doi: 10.1002/anie.200906147

Author Information

  1. 1

    Institut für Strukturbiologie, Helmholtz Zentrum München http://www.helmholtz-muenchen.de/stb

  2. 2

    Munich Center for Integrated Protein Science at Department Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany), Fax: (+49) 89-289-13867

  3. 3

    European Molecular Biology Laboratory, Heidelberg (Germany)

  4. 4

    Institut Européen de Chimie et Biologie, Pessac (France)

  5. 5

    Institut Pasteur, NRS URA 2185, Paris (France)

  1. †

    We thank Frank Gabel for discussions. C.D.M. and T.M. acknowledge support by EMBO Long Term Fellowships. T.M. thanks the Austrian Science Fund (FWF) for a Schrödinger Fellowship. We thank the EU NMR LSF, Frankfurt and the Bavarian NMR Centre (BNMRZ), Garching for NMR measurement time. This work was supported by the European Commission, grants 3D Repertoire and FSG-V-RNA (M.S.) and Deutsche Forschungsgemeinschaft (Sa 823/3).

Publication History

  1. Issue published online: 3 MAR 2010
  2. Article first published online: 10 FEB 2010
  3. Manuscript Revised: 21 DEC 2009
  4. Manuscript Received: 1 NOV 2009

Funded by

  • EU NMR LSF, Frankfurt
  • Bavarian NMR Centre (BNMRZ), Garching
  • European Commission
  • Deutsche Forschungsgemeinschaft

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Keywords:

  • NMR spectroscopy;
  • paramagnetic relaxation enhancement;
  • protein structures;
  • residual dipolar coupling;
  • structural biology

Graphical Abstract

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Putting the pieces together: An efficient, generally applicable approach for structural analysis of protein complexes and multidomain proteins in solution based on NMR spectroscopy is presented. Starting from available high-resolution structures of individual domains or subunits, the overall domain arrangement is calculated from NMR spectroscopy data that can be obtained for high-molecular-weight complexes.

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