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Single-Molecule Kinetics of Two-Step Divalent Cation Chelation

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  • Supported by the MRC.

Abstract

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A double hug: Two half-chelating ligands were covalently attached within the lumen of a protein nanopore (see picture). By electrical recording, the formation of fully chelated Zn2+ ions was monitored at the single-molecule level, thus revealing the rate constants for the eight major steps in the process.

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