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Characterization of Picomole Amounts of Oligosaccharides from Glycoproteins by 1H NMR Spectroscopy

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  • We acknowledge a grant from the DFG for the 700 MHz NMR Spectrometer and thank Prof. Dr. H. Weller, Hamburg, for access to a plasma cleaner.

Abstract

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Can you see it? NMR spectroscopy is indispensible for structure analysis. It is believed that its use is limited if less than several nanomoles are available. The characterization of oligosaccharides as components of glycoproteins by NMR spectroscopy utilizing significantly less material is highly desirable. Optimization of the sample preparation, water suppression, and instrument setup has enabled only 15 picomoles of oligosaccharides to be analyzed by NMR spectroscopy.

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