The Ames Laboratory is operated for the US Department of Energy by Iowa State University under Contract DE-AC02-07CH11358. This research was supported by the Director of Science, Office of Basic Energy Sciences, Division of Chemical Sciences and partially supported by research funds of Chonbuk National University in 2008.
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Communication
Detection of Single Enzyme Molecules inside Nanopores on the Basis of Chemiluminescence†
Article first published online: 8 MAR 2010
DOI: 10.1002/anie.200906713
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Kang, S., Lee, S. and Yeung, E. (2010), Detection of Single Enzyme Molecules inside Nanopores on the Basis of Chemiluminescence. Angewandte Chemie International Edition, 49: 2603–2606. doi: 10.1002/anie.200906713
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Publication History
- Issue published online: 23 MAR 2010
- Article first published online: 8 MAR 2010
- Manuscript Revised: 4 FEB 2010
- Manuscript Received: 28 NOV 2009
Funded by
- US Department of Energy. Grant Number: DE-AC02-07CH11358
Keywords:
- analytical methods;
- chemiluminescence;
- enzymes;
- nanopores;
- single-molecule studies
Graphical Abstract
Trapped, but not immobilized inside 50 nm membrane pores, luciferase was detected on the basis of chemiluminescence (see scheme) with an intensified-charge-coupled-device (ICCD) camera. Light emission from the nanopores was concentrated onto individual pixels of the ICCD. Single-molecule detection was possible by integration of the amplified signal over a prolonged enzyme reaction time.