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Detection of Single Enzyme Molecules inside Nanopores on the Basis of Chemiluminescence


  • The Ames Laboratory is operated for the US Department of Energy by Iowa State University under Contract DE-AC02-07CH11358. This research was supported by the Director of Science, Office of Basic Energy Sciences, Division of Chemical Sciences and partially supported by research funds of Chonbuk National University in 2008.


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Trapped, but not immobilized inside 50 nm membrane pores, luciferase was detected on the basis of chemiluminescence (see scheme) with an intensified-charge-coupled-device (ICCD) camera. Light emission from the nanopores was concentrated onto individual pixels of the ICCD. Single-molecule detection was possible by integration of the amplified signal over a prolonged enzyme reaction time.