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Detection of Single Enzyme Molecules inside Nanopores on the Basis of Chemiluminescence

Authors


  • The Ames Laboratory is operated for the US Department of Energy by Iowa State University under Contract DE-AC02-07CH11358. This research was supported by the Director of Science, Office of Basic Energy Sciences, Division of Chemical Sciences and partially supported by research funds of Chonbuk National University in 2008.

Abstract

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Trapped, but not immobilized inside 50 nm membrane pores, luciferase was detected on the basis of chemiluminescence (see scheme) with an intensified-charge-coupled-device (ICCD) camera. Light emission from the nanopores was concentrated onto individual pixels of the ICCD. Single-molecule detection was possible by integration of the amplified signal over a prolonged enzyme reaction time.

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