The work was supported by the EU-funded project EU-NMR. The Center for Biomolecular Magnetic Resonance is funded by the state of Hesse. H.S. and C.G. are members of the DFG-funded Cluster of Excellence “Makromoleculare Komplexe”.
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Communication
High-Resolution Studies of Uniformly 13C,15N-Labeled RNA by Solid-State NMR Spectroscopy†
Article first published online: 8 JUN 2010
DOI: 10.1002/anie.200906885
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Cherepanov, Alexey V., Glaubitz, C. and Schwalbe, H. (2010), High-Resolution Studies of Uniformly 13C,15N-Labeled RNA by Solid-State NMR Spectroscopy. Angew. Chem. Int. Ed., 49: 4747–4750. doi: 10.1002/anie.200906885
- †
Publication History
- Issue published online: 21 JUN 2010
- Article first published online: 8 JUN 2010
- Manuscript Revised: 16 FEB 2010
- Manuscript Received: 7 DEC 2009
Funded by
- EU
- DFG
Keywords:
- conformation analysis;
- freeze-quenching;
- NMR spectroscopy;
- RNA;
- solid-state structures
Chilling out: Solid-state 13C NMR correlation spectroscopy was used to assign the signals of a uniformly labeled RNA hairpin in frozen aqueous solution. Conformational analysis shows that solutions of biologically relevant RNAs can freeze without significant changes in RNA structure and without critical loss of resolution and sensitivity in NMR experiments.