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Rhodamines NN: A Novel Class of Caged Fluorescent Dyes


  • This work was supported by the Leibniz Prize fund of the DFG (to SWH) and the Max-Planck-Gesellschaft. We are grateful to Donald Ouw (MPI), Reinhard Machinek, Dr. Holm Frauendorf, and their co-workers (Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen) for recording spectra.


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A bright future: The reaction of diazomethane with acid chlorides obtained from N,N,N′,N′-tetraalkylrhodamines affords 2-diazo-2,3-dihydro-1H-indenespiro[1,9′]-9H-xanthen-3-ones—a novel class of caged rhodamines. These dyes allow new imaging procedures based on the step-wise activation and detection of several fluorescent markers (see picture).