These authors contributed equally to this paper.
DNA-Templated Synthesis of Trimethine Cyanine Dyes: A Versatile Fluorogenic Reaction for Sensing G-Quadruplex Formation†
Article first published online: 12 MAR 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 15, pages 2738–2742, April 1, 2010
How to Cite
Meguellati, K., Koripelly, G. and Ladame, S. (2010), DNA-Templated Synthesis of Trimethine Cyanine Dyes: A Versatile Fluorogenic Reaction for Sensing G-Quadruplex Formation. Angew. Chem. Int. Ed., 49: 2738–2742. doi: 10.1002/anie.201000291
S.L. thanks the International Centre for Frontier Research in Chemistry (icFRC) for financial support.
- Issue published online: 26 MAR 2010
- Article first published online: 12 MAR 2010
- Manuscript Received: 18 JAN 2010
- International Centre for Frontier Research in Chemistry (icFRC)
- fluorescent probes;
- peptide nucleic acids
A healthy glow: Fluorogenic peptide nucleic acids (PNAs) functionalized with indoline derivatives are used to specifically sense G-quadruplex formation. Upon hybridization of both PNAs to the single-stranded flanking arms of quadruplex DNA (see scheme), the synthesis of a trimethine cyanine dye is templated. Dye formation can be detected by the appearance of a characteristic fluorescence signal.