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Probing Secondary Structures of Spin-Labeled RNA by Pulsed EPR Spectroscopy


  • Financial support from the Max Planck Society is gratefully acknowledged.


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Tempo, TEMPO! Spin-labeled RNAs are readily prepared by postsynthetic modification of RNA. This approach provides access to paramagnetic oligonucleotides with position-specific probes in cytidine, adenosine, and guanosine nucleobases (CT, AT, GT, respectively). In this way alternative RNA secondary structures can be investigated by distance measurements using pulsed EPR spectroscopy.