Communication

2-Aminopurine Incorporation Perturbs the Dynamics and Structure of DNA†

  1. André Dallmann Dr.2,
  2. Lars Dehmel2,
  3. Torben Peters2,
  4. Clemens Mügge Dr.2,
  5. Christian Griesinger Dr.1,
  6. Jennifer Tuma Dr.1,
  7. Nikolaus P. Ernsting Dr.2

Article first published online: 14 JUL 2010

DOI: 10.1002/anie.201001312

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 49, Issue 34, pages 5989–5992, August 9, 2010

Additional Information

How to Cite

Dallmann, A., Dehmel, L., Peters, T., Mügge, C., Griesinger, C., Tuma, J. and Ernsting, Nikolaus P. (2010), 2-Aminopurine Incorporation Perturbs the Dynamics and Structure of DNA. Angew. Chem. Int. Ed., 49: 5989–5992. doi: 10.1002/anie.201001312

Author Information

  1. 1

    Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen (Germany)

  2. 2

    Humboldt Universität zu Berlin, Institut für Chemie, 12489 Berlin (Germany)

  1. †

    We thank the Humboldt University (N.P.E.) and the Max-Planck-Society (C.G.) for support. This work was funded by the DFG under ER 154/9-1 (N.P.E.) and GR 1211/14-1 (C.G.). We thank Dr. H. Schmidt, Göttingen, for help with the phage preparation and useful discussions and Dr. C. Schwieters for help with the implementation of methyl RDCs into structure calculations.

Publication History

  1. Issue published online: 30 JUL 2010
  2. Article first published online: 14 JUL 2010
  3. Manuscript Received: 4 MAR 2010

Funded by

  • DFG

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Keywords:

  • 2-aminopurine;
  • base-pair lifetimes;
  • DNA;
  • helical structures;
  • NMR spectroscopy
Thumbnail image of graphical abstract

Vive la petite différence: 2-Aminopurine (2AP) induces small but detectable perturbations of the DNA helix. More significant are changes in the base-pair dynamics in the vicinity of 2AP. The differences have been characterized in NMR and UV spectroscopic studies on a 13-mer DNA double strand with either adenine (A) or 2AP in a central position.

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