Communication

Quantitative CARS Molecular Fingerprinting of Single Living Cells with the Use of the Maximum Entropy Method†

  1. Masanari Okuno1,
  2. Prof. Dr. Hideaki Kano1,
  3. Prof. Dr. Philippe Leproux3,
  4. Prof. Dr. Vincent Couderc3,
  5. Dr. James P. R. Day4,
  6. Prof. Dr. Mischa Bonn4,
  7. Prof. Dr. Hiro-o Hamaguchi1,2

Article first published online: 2 AUG 2010

DOI: 10.1002/anie.201001560

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 49, Issue 38, pages 6773–6777, September 10, 2010

Additional Information

How to Cite

Okuno, M., Kano, H., Leproux, P., Couderc, V., Day, James P. R., Bonn, M. and Hamaguchi, H.-o. (2010), Quantitative CARS Molecular Fingerprinting of Single Living Cells with the Use of the Maximum Entropy Method. Angew. Chem. Int. Ed., 49: 6773–6777. doi: 10.1002/anie.201001560

Author Information

  1. 1

    Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1 Bunkyo-ku Tokyo, 113-0033 (Japan), Fax: (+81) 3818-4621

  2. 2

    Institute of Molecular Science, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 300 (Taiwan)

  3. 3

    Institut de Recherche XLIM, UMR CNRS, No. 6172, 123 avenue Albert Thomas, 87060 Limoges cedex (France)

  4. 4

    Biosurface Spectroscopy, FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam (The Netherlands)

  1. †

    This work was supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support from the Precursory Research for Embryonic Science and Technology (PRESTO) program of JST. The authors thank C. Onogi for providing the spontaneous Raman spectrum of yeast mitochondria, Leukos and Horus Laser companies for technical support, and Dr. F. Omura and H. Yomo (Suntory Co., Ltd.) for providing us with the yeast sample. We gratefully acknowledge J. Ukon (HORIBA, Ltd.) for assisting in the collaboration between the Japanese and French groups.

Publication History

  1. Issue published online: 7 SEP 2010
  2. Article first published online: 2 AUG 2010
  3. Manuscript Revised: 27 MAY 2010
  4. Manuscript Received: 16 MAR 2010

Funded by

  • Japan Science and Technology Agency
  • JST

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Keywords:

  • CARS (coherent anti-Stokes Raman scattering);
  • time-resolved spectroscopy;
  • vibrational spectroscopy
Thumbnail image of graphical abstract

Fast CARS: The cell-death process was observed in real time at the subcellular level by coherent anti-Stokes Raman microspectroscopy, an extension of anti-Stokes Raman scattering (CARS) microscopy. Changes in the chemical contrast during the dying process can be clearly resolved (see picture).

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