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A Cleavable Linker Based on the Levulinoyl Ester for Activity-Based Protein Profiling

Authors

  • Paul P. Geurink,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Dr. Bogdan I. Florea,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Nan Li,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Dr. Martin D. Witte,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Joeri Verasdonck,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Chi-Lin Kuo,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Prof. Dr. Gijs A. van der Marel,

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Prof. Dr. Herman S. Overkleeft

    1. Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden (The Netherlands), Fax: (+31) 71-527-4307
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  • Financial support for this work was provided by the Netherlands Organisation for Scientific Research (NWO) and the Netherlands Genomics Initiative (NGI).

Abstract

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Get linked: The title linker is stable under various biological conditions and can be cleaved chemoselectively with hydrazine (see scheme). Its use is demonstrated in the activity-based enrichment and identification of proteasome active subunits from cell extracts.

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