High-Throughput Method for Determining the Enantioselectivity of Enzyme-Catalyzed Hydroxylations Based on Mass Spectrometry


  • This work was supported by the Science & Engineering Research Council of A*STAR, Singapore, through a research grant (project No. 0621010024). J.M. thanks the China Scholarship Council for a scholarship.


original image

Up to speed: An accurate, sensitive, high-throughput, and simple method for measuring the product ee value of enzyme-catalyzed hydroxylations (see scheme) is based on the use of enantiopure or enantioenriched deuterated substrates and mass spectrometric detection.