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Quantification of the Sixth DNA Base Hydroxymethylcytosine in the Brain

Authors

  • Martin Münzel Dipl.-Chem.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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    • These authors contributed equally to this work.

  • Daniel Globisch Dipl.-Chem.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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    • These authors contributed equally to this work.

  • Tobias Brückl Dipl.-Chem.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • Mirko Wagner Dipl.-Chem.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • Veronika Welzmiller Dipl.-Chem.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • Stylianos Michalakis Dr.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Pharmacy, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • Markus Müller Dr.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • Martin Biel Prof. Dr.,

    1. Center for Integrated Protein Science (CiPSM) at the Department of Pharmacy, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • Thomas Carell Prof. Dr.

    1. Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Butenandtstrasse 5–13, 81377 Munich (Germany)
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  • We thank the excellence cluster CiPSM, SFB 646, and SFB 749 for generous support. M. Münzel is grateful to the Fonds of the Chemical Industry for a Kekulé fellowship. We thank V. Hammelmann for support during this project.

Abstract

original image

Mind over matter: LC-MS has allowed the amount of the post-replicatively formed DNA base 5-hydroxymethylcytosine (see picture; left) to be quantified in brain tissue. The nucleoside is most abundant in areas that are associated with higher cognitive functions, and its content in mouse hippocampi seems to increase with age. The new method enables hydroxymethylcytosine to be quantified with unprecedented accuracy.

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