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An Artificial Metalloenzyme: Creation of a Designed Copper Binding Site in a Thermostable Protein


  • This work was supported by the Deutsche Forschungsgemeinschaft (Schwerpunkt 1170). We thank R. Sterner for providing the tHisF plasmid. We also thank E. Kravets for her work on the initial mutants, as well as H. Hinrichs and D. Klütt for HPLC support.


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Guided by nature: A designed binding site comprising the His/His/Asp motif for CuII complexation has been constructed in a robust protein by site-specific mutagenesis (see picture). The artificial metalloenzyme catalyzes an enantioselective Diels–Alder reaction.