These authors contributed equally to the work and are listed alphabetically.
Probing the Active Site of an O2-Tolerant NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 by In Situ EPR and FTIR Spectroscopy†
Article first published online: 20 SEP 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 43, pages 8026–8029, October 18, 2010
How to Cite
Horch, M., Lauterbach, L., Saggu, M., Hildebrandt, P., Lendzian, F., Bittl, R., Lenz, O. and Zebger, I. (2010), Probing the Active Site of an O2-Tolerant NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 by In Situ EPR and FTIR Spectroscopy. Angew. Chem. Int. Ed., 49: 8026–8029. doi: 10.1002/anie.201002197
The work was supported by the DFG (SFB498 and Cluster of Excellence “Unicat”). The authors are indebted to Bärbel Friedrich and Siem Albracht for critical comments and helpful discussions.
- Issue published online: 13 OCT 2010
- Article first published online: 20 SEP 2010
- Manuscript Revised: 17 JUN 2010
- Manuscript Received: 14 APR 2010
- EPR spectroscopy;
- FTIR spectroscopy;
- oxygen tolerance
A clear picture: In situ EPR and FTIR spectroscopic studies on the soluble, NAD+-reducing [NiFe]-hydrogenase of Ralstonia eutropha reveal that the catalytic site resides predominantly in the intermediate Nia-C state within whole cells. This state, can either be reversibly oxidized to a “Nir-B”-like state or further reduced to various Nia-SR species. The data suggest that the iron center in the active site contains a standard set (one CO and two CN−) of inorganic ligands.