This work was supported by the Icelandic Research Fund (080041022) and the University of Iceland Research Fund, including a doctoral fellowship to S.A.S.
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Communication
Noncovalent and Site-Directed Spin Labeling of Nucleic Acids†
Article first published online: 15 SEP 2010
DOI: 10.1002/anie.201002637
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Shelke, S. A. and Sigurdsson, S. Th. (2010), Noncovalent and Site-Directed Spin Labeling of Nucleic Acids. Angew. Chem. Int. Ed., 49: 7984–7986. doi: 10.1002/anie.201002637
- †
Publication History
- Issue published online: 13 OCT 2010
- Article first published online: 15 SEP 2010
- Manuscript Revised: 7 JUL 2010
- Manuscript Received: 2 MAY 2010
Funded by
- Icelandic Research Fund. Grant Number: 080041022
- University of Iceland Research Fund
Keywords:
- DNA;
- EPR spectroscopy;
- nucleic acids;
- receptor–ligand interactions;
- spin labels
Spin label parking: The rigid spin label ç (magenta), which is an analogue of cytidine, binds site-specifically to abasic sites in duplex DNAs, through hydrogen bonding to guanine (blue) and π stacking with the flanking base pairs. EPR spectroscopy shows complete binding of ç to the abasic site at low temperatures. This new spin-labeling strategy gives easy access to spin-labeled nucleic acids for structural studies by pulsed EPR techniques.