Amplified Aptamer-Based Assay through Catalytic Recycling of the Analyte

Authors

  • Chun-Hua Lu,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Juan Li,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Mei-Hua Lin,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Yi-Wei Wang,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Prof. Huang-Hao Yang,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Prof. Xi Chen,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Prof. Guo-Nan Chen

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • This research was supported by the National Natural Science Foundation of China (No. 20735002, No. 20975023), the National Basic Research Program of China (No. 2010CB732403), and the “Program for New Century Excellent Talents in University” of China.

Abstract

original image

Turn up the volume: In a simple and highly sensitive aptamer-based assay that relies on the ability of nanomaterials to protect DNA from nuclease cleavage, the aptamer is released from the nanomaterial when challenged with a target. The nuclease then cleaves the free aptamer and releases the target, which can initiate a new cycle. Recycling of the target in this way leads to significant signal amplification (see diagram).

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