A Nanoparticle Size Series for In Vivo Fluorescence Imaging

Authors

  • Dr. Zoran Popović,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Dr. Wenhao Liu,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Vikash P. Chauhan,

    1. Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston MA (USA)
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  • Jungmin Lee,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Cliff Wong,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Dr. Andrew B. Greytak,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Numpon Insin,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Prof. Dr. Daniel G. Nocera,

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • Prof. Dr. Dai Fukumura,

    1. Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston MA (USA)
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  • Prof. Dr. Rakesh K. Jain,

    1. Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston MA (USA)
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  • Prof. Dr. Moungi G. Bawendi

    1. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139-4307 (USA), Fax: (+1) 617-452-2708
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  • This research was supported by the U.S. National Cancer Institute grants R01-CA126642 (R.K.J., D.F., M.G.B., and D.G.N.), R01-CA085140, R01-CA115767 (R.K.J.), P01-CA080124 (R.K.J. and D.F.), R01-CA096915 (D.F.); by the MIT-Harvard NIH Center for Cancer Nanotechnology Excellence (1U54-CA119349) (M.G.B.); by the MIT DCIF (CHE-980806, DBI-9729592); by the ISN (W911NF-07-D-0004) (M.G.B and D.G.N); by the NSF-MRSEC program (DMR-0117795) via the use of its shared user facilities; and by the NSF-CRS (CHE-0714189). W.L. was supported by a NSF Graduate Research Fellowship. A.B.G. was a Novartis Fellow of the Life Sciences Research Foundation. We would like to thank S. Geyer for valuable help with quantum yield measurements and to J. Cui for the help with FCS measurements.

Abstract

original image

A nanoparticle toolset was created within the size limits of 10–150 nm for probing size-dependent nanoparticle distribution in solid tumors. By using multiphoton intravital microscopy, the particles were tracked both spatially and temporally in the same tumor grown in a transparent window model.

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