Picking a pucker! Fixed-conformation North (N)- and South (S)-methanocarba-2′-deoxyadenosine-triphosphate (MC-dATP) have different effects in DNA strand extension catalyzed by HIV-1 RT and three human Y-family DNA polymerases (pols). All of the polymerases tested preferred the N-oriented furanose geometry, though pol η could insert both pucker mimics. The growth of malignant breast cancer cells overexpressing pol ι was more severely inhibited by N-MC-dATP than that of nonmalignant breast cells.
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