These authors contributed equally.
A Straightforward Approach for Cellular-Uptake Quantification†
Article first published online: 23 SEP 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 44, pages 8240–8243, October 25, 2010
How to Cite
Paramelle, D., Subra, G., Vezenkov, L. L., Maynadier, M., André, C., Enjalbal, C., Calmès, M., Garcia, M., Martinez, J. and Amblard, M. (2010), A Straightforward Approach for Cellular-Uptake Quantification. Angew. Chem. Int. Ed., 49: 8240–8243. doi: 10.1002/anie.201003347
This study was supported in part by the French National Agency (ANR) (ANR-08-BLAN-0066-01) and a grant from the Università di Napoli Federico II, Italy (L.L.V.).
- Issue published online: 20 OCT 2010
- Article first published online: 23 SEP 2010
- Manuscript Revised: 30 JUL 2010
- Manuscript Received: 2 JUN 2010
- French National Agency (ANR). Grant Number: ANR-08-BLAN-0066-01
- Università di Napoli Federico II, Italy
- cell-penetrating peptides;
- cellular uptake;
- isotopic labeling;
- mass spectrometry;
Putting a number on it: MALDI-TOF MS enabled direct quantification of the cellular uptake of cell-penetrating peptides (CPPs) by MDA-MB-231 breast cancer cells. This sensitive general strategy (see schematic representation), which requires no purification or separation steps, relies on the enhancement and discrimination of the MS signals of an α-cyano-4-hydroxycinnamic acid tag in a neutral α-cyano-4-hydroxycinnamic methyl ester matrix.