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A Straightforward Approach for Cellular-Uptake Quantification


  • This study was supported in part by the French National Agency (ANR) (ANR-08-BLAN-0066-01) and a grant from the Università di Napoli Federico II, Italy (L.L.V.).


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Putting a number on it: MALDI-TOF MS enabled direct quantification of the cellular uptake of cell-penetrating peptides (CPPs) by MDA-MB-231 breast cancer cells. This sensitive general strategy (see schematic representation), which requires no purification or separation steps, relies on the enhancement and discrimination of the MS signals of an α-cyano-4-hydroxycinnamic acid tag in a neutral α-cyano-4-hydroxycinnamic methyl ester matrix.