Funding by the Austrian Science Foundation FWF (P21640, I317) and the Ministry of Science and Research bm:wf (GenAU III, “Non-coding RNA” P0726-012-012) is acknowledged. M.A. thanks the Austrian Academy of Science and the UNESCO for a L′ORÉAL fellowship.
Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′-Azido-3′-Deoxyadenosine RNA†
Article first published online: 31 AUG 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 41, pages 7470–7472, October 4, 2010
How to Cite
Steger, J., Graber, D., Moroder, H., Geiermann, A.-S., Aigner, M. and Micura, R. (2010), Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′-Azido-3′-Deoxyadenosine RNA. Angew. Chem. Int. Ed., 49: 7470–7472. doi: 10.1002/anie.201003424
- Issue published online: 29 SEP 2010
- Article first published online: 31 AUG 2010
- Manuscript Received: 5 JUN 2010
- Austrian Science Foundation FWF. Grant Numbers: P21640, I317, Ministry of Science and Research bm:wf, GenAU III
- solid-phase synthesis;
Flexibility exercised: Hydrolysis-resistant 3′-aminoacyl-tRNA conjugates that contain a stable amide linkage instead of the natural ester are valuable substrates for biochemical studies of ribosomal processes. In a novel preparation of the stable E. coli initiator tRNA derivative 3′-(N-formylmethionyl)amino-tRNAfMet the key feature is the synthesis of 3′-azido oligoribonucleotides using a new functionalized solid support.