SEARCH

SEARCH BY CITATION

Keywords:

  • computational chemistry;
  • isopeptide bonds;
  • NMR spectroscopy;
  • protein structures
Thumbnail image of graphical abstract

One bond makes all the difference: Three suitably positioned amino acid side chains (see picture) and a hydrophobic environment are all that is required for an amidation reaction with remarkable consequences. An emerging central building block of bacterial surface proteins owes its stability to a spontaneously formed isopeptide bond. The impact of this bond on protein structure and dynamics and the mechanism of its formation are scrutinized in detail.