This work was supported by the EU DNAREC Marie Curie Training Site (MEST-CT-2005-020842, C.D. and A.L.). We thank Rachel Kershaw for supplying the mouse cDNA and Prof. J. Kevin Chipman for helpful discussions.
Noncovalent DNA-Binding Metallo-Supramolecular Cylinders Prevent DNA Transactions in vitro†
Article first published online: 8 OCT 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 49, Issue 47, pages 8942–8945, November 15, 2010
How to Cite
Ducani, C., Leczkowska, A., Hodges, N. J. and Hannon, M. J. (2010), Noncovalent DNA-Binding Metallo-Supramolecular Cylinders Prevent DNA Transactions in vitro. Angew. Chem. Int. Ed., 49: 8942–8945. doi: 10.1002/anie.201004471
- Issue published online: 11 NOV 2010
- Article first published online: 8 OCT 2010
- Manuscript Received: 21 JUL 2010
- EU. Grant Number: MEST-CT-2005-020842
- helical structures;
- PCR inhibition;
- supramolecular chemistry
Supramolecular metal cylinders not only bind noncovalently to DNA inducing substantial coiling but are able to recognize specific DNA structures involved in biological processes as DNA replication. It is demonstrated that these molecules can inhibit the amplification of the DNA mediated by a Taq DNA polymerase and are even able to displace the enzyme from a double strand DNA (see scheme).