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Noncovalent DNA-Binding Metallo-Supramolecular Cylinders Prevent DNA Transactions in vitro

Authors

  • Cosimo Ducani,

    1. School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B152TT (UK), Fax: (+44) 121-414-7871
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  • Anna Leczkowska,

    1. School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B152TT (UK), Fax: (+44) 121-414-7871
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  • Dr. Nikolas J. Hodges,

    Corresponding author
    1. School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B152TT (UK), Fax: (+44) 121-414-5925
    • School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B152TT (UK), Fax: (+44) 121-414-5925
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  • Prof. Michael J. Hannon

    Corresponding author
    1. School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B152TT (UK), Fax: (+44) 121-414-7871
    • School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B152TT (UK), Fax: (+44) 121-414-7871
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  • This work was supported by the EU DNAREC Marie Curie Training Site (MEST-CT-2005-020842, C.D. and A.L.). We thank Rachel Kershaw for supplying the mouse cDNA and Prof. J. Kevin Chipman for helpful discussions.

Abstract

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Supramolecular metal cylinders not only bind noncovalently to DNA inducing substantial coiling but are able to recognize specific DNA structures involved in biological processes as DNA replication. It is demonstrated that these molecules can inhibit the amplification of the DNA mediated by a Taq DNA polymerase and are even able to displace the enzyme from a double strand DNA (see scheme).

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