Pure Protein Microspheres by Calcium Carbonate Templating

Authors

  • Dr. Dmitry V. Volodkin,

    Corresponding author
    1. Stranski Laboratorium für Physikalische und Theoretische Chemie, Sekr. TC 9, Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 124, 10623 Berlin (Germany)
    • Stranski Laboratorium für Physikalische und Theoretische Chemie, Sekr. TC 9, Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 124, 10623 Berlin (Germany)
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  • Prof. Dr. Regine von Klitzing,

    1. Stranski Laboratorium für Physikalische und Theoretische Chemie, Sekr. TC 9, Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 124, 10623 Berlin (Germany)
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  • Prof. Dr. Helmuth Möhwald

    1. Department of Interfaces, Max Planck Institute for Colloids and Interfaces, Am Mühlenberg 1, Golm/Potsdam, 14424 (Germany)
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  • D.V.V. thanks the Alexander von Humboldt Foundation for support (AvH Fellowship). We also thank Rona Pitschke and Heike Runge for SEM and TEM imaging.

Abstract

original image

Swapping spheres: Monodisperse protein microspheres can be fabricated by templating on porous CaCO3 microcores (see picture; CaCO3 gray, insulin orange). The CaCO3 cores decompose upon variation of pH from 9.0 to 5.0, whereas the insulin model protein precipitates. The method requires mild conditions, no additives, and minimal processing.

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