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Two-Dimensional Ultraviolet (2DUV) Spectroscopic Tools for Identifying Fibrillation Propensity of Protein Residue Sequences


  • This research was supported by the National Institutes of Health (grants GM059230 and GM091364) and the National Science Foundation (grant CHE-0745892). We thank Prof. Yi Luo for helpful discussions.


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Distinctive feature: Two-dimensional ultraviolet (2DUV) spectroscopy can be utilized to identify protein sequences capable of forming fibrils. The Rosetta free energy of proteins, which is a good indicator of fibrillation propensity, has distinct 2DUV signatures. An additional positive diagonal peak at 54 000–58 000 cm−1 in chiral xxxy spectra serves as a indicator of the ability of protein sequences to form amyloid-like fibrils.