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Amplified Detection of DNA through an Autocatalytic and Catabolic DNAzyme-Mediated Process


  • Financial support by the EU ECCell project, the Israel Science Foundation (Converging Technologies fellowship to J.E.), and the Minerva foundation (postdoctoral fellowship to C.T.) is gratefully acknowledged. We thank Prof. G. von Kiedrowski for helpful comments.


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Sense and sensibility: Amplified DNA detection is accomplished by the autocatalytic cleavage of a programmed nucleic acid structure based on the Mg2+-dependent DNAzyme E6 (see picture). The analyte sequence is autocatalytically generated by the DNAzyme from a reporter nucleic acid, which is labeled with a fluorophore and quencher at its two ends.