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Single-Molecule Four-Color FRET

Authors

  • Jinwoo Lee ,

    1. Department of Physics and Astronomy, Department of Biophysics and Chemical Biology, National Center for Creative Research Initiatives, Seoul National University, Seoul 151-747 (Korea), Fax: (+82) 2-871-8083
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    • These authors contributed equally to this work.

  • Sanghwa Lee ,

    1. Department of Physics and Astronomy, Department of Biophysics and Chemical Biology, National Center for Creative Research Initiatives, Seoul National University, Seoul 151-747 (Korea), Fax: (+82) 2-871-8083
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    • These authors contributed equally to this work.

  • Kaushik Ragunathan,

    1. Center for Biophysics and Computational Biology, Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801 (USA)
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  • Dr. Chirlmin Joo,

    1. School of Biological Sciences, Seoul National University, Seoul 151-747 (Korea)
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  • Prof. Dr. Taekjip Ha,

    1. Center for Biophysics and Computational Biology, Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Howard Hughes Medical Institute, Urbana, IL 61801 (USA)
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  • Prof. Dr. Sungchul Hohng

    Corresponding author
    1. Department of Physics and Astronomy, Department of Biophysics and Chemical Biology, National Center for Creative Research Initiatives, Seoul National University, Seoul 151-747 (Korea), Fax: (+82) 2-871-8083
    • Department of Physics and Astronomy, Department of Biophysics and Chemical Biology, National Center for Creative Research Initiatives, Seoul National University, Seoul 151-747 (Korea), Fax: (+82) 2-871-8083
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  • This work was supported by the Creative Research Initiatives (Physical Genetics Laboratory, 2009-0081562), the World Class University project (R31-2009-100320), and the US National Science Foundation grants (0822613 and 0646550). T.H. is an investigator with the Howard Hughes Medical Institute. J.L. was financially supported by a Hi Seoul Science (Humanities) Fellowship from Seoul Scholarship Foundation.

Abstract

original image

Six interfluorophore FRET efficienciesEij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.

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