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Insights into the Finer Issues of Native Chemical Ligation: An Approach to Cascade Ligations

Authors

  • Dr. Zhongping Tan,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • Dr. Shiying Shang,

    1. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
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  • Prof. Samuel J. Danishefsky

    Corresponding author
    1. Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027 (USA), Fax: (+1) 212-772-8691
    2. Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
    • Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027 (USA), Fax: (+1) 212-772-8691
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  • Support for this research was provided by the National Institutes of Health (CA28824 to S.J.D.).

Abstract

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Maximizing by minimizing: An efficient and broadly useful two-step ligation protocol was developed. Important mechanistic issues of ligation were probed from competition studies on the formation of diastereomeric ligation products. This study provides a valuable approach to facilitate polypeptide synthesis by minimizing protecting group manipulations and intermediate isolations.

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