Highly Efficient Capture of Circulating Tumor Cells by Using Nanostructured Silicon Substrates with Integrated Chaotic Micromixers

Authors

  • Dr. Shutao Wang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing (P. R. China)
    2. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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    • These authors contributed equally to this work.

  • Dr. Kan Liu,

    1. College of Electronics and Information Engineering, Wuhan Textile University, Wuhan (P. R. China)
    2. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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    • These authors contributed equally to this work.

  • Dr. Jian Liu,

    1. Uro-oncology Research Program, Samuel Oschin Comprehensive Cancer Institute, Cedars Sinai Medical Center, Los Angeles (USA)
    2. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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    • These authors contributed equally to this work.

  • Dr. Zeta T.-F. Yu,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Xiaowen Xu,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Libo Zhao,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Tom Lee,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Dr. Eun Kyung Lee,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Jean Reiss,

    1. Department of Pathology and Laboratory Medicine, University of California, Los Angeles (USA)
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  • Prof. Yi-Kuen Lee,

    1. Department of Mechanical Engineering, The Hong Kong University of Science and Technology (Hong Kong)
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  • Prof. Leland W. K. Chung,

    1. Uro-oncology Research Program, Samuel Oschin Comprehensive Cancer Institute, Cedars Sinai Medical Center, Los Angeles (USA)
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  • Prof. Jiaoti Huang,

    1. Department of Pathology and Laboratory Medicine, University of California, Los Angeles (USA)
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  • Prof. Matthew Rettig,

    1. Department of Urology, University of California, Los Angeles (USA)
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  • Prof. David Seligson,

    1. Department of Pathology and Laboratory Medicine, University of California, Los Angeles (USA)
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  • Dr. Kumaran N. Duraiswamy,

    Corresponding author
    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
    • Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Prof. Clifton K.-F. Shen,

    Corresponding author
    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
    • Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • Prof. Hsian-Rong Tseng

    Corresponding author
    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
    • Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1) 310-206-8975 http://labs.pharmacology.ucla.edu/tsenglab/
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  • This research was supported by the NIH IMAT Program (R21A151159-01) and a Prostate Cancer Foundation Creativity Award. We thank Prof. Allan Pantuck from the UCLA Urology Department for providing CTC blood samples.

Abstract

original image

Finding a needle in a haystack: A new technology is demonstrated to enrich circulating tumor cells (CTCs) with high efficiency by integrating an antibody-coated silicon nanopillar (SiNP, see picture; gray) substrate with an overlaid polydimethylsiloxane (PDMS) microfluidic chaotic mixer (turquoise). It shows significantly improved sensitivity in detecting rare CTCs from whole blood, thus providing an alternative for monitoring cancer progression.

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