Orthogonal Protein Decoration of DNA Origami

Authors

  • Dr. Barbara Saccà,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dipl.-Chem. Rebecca Meyer,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dipl.-Biotechnol. Michael Erkelenz,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
  • M. Sc. Kathrin Kiko,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Andreas Arndt,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Dr. Hendrik Schroeder,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Dr. Kersten S. Rabe,

    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Prof. Christof M. Niemeyer

    Corresponding author
    1. Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    • Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund (Germany), Fax: (+49) 231-755-7082
    Search for more papers by this author

  • This work was supported by the Deutsche Forschungsgemeinschaft (SA 1952/1-1, NI 399/10-1) and the Max-Planck Society (fellowship to C.M.N.). We are most grateful to Prof. Kai Johnsson for the generous donation of the covalin expression plasmid and helpful discussions. We also thank Prof. Mathias Sprinzl for kind donation of a plasmid encoding for EST2, and Dr. Michael Adler for help with IPCR analyses. Stephanie Reisewitz and Julian Engel are acknowledged for experimental support in cloning.

Abstract

original image

If the face fits: Self-labeling fusion proteins have been used for the site-specific decoration of DNA origami. This method even allows individual faces of the quasi-two-dimensional plane of the nanostructure to be specifically decorated (see picture), thereby enabling directional immobilization and thus control over the accessibility of distinct proteins presented on the structure.

Ancillary