Far-Field Nanoscopy with Reversible Chemical Reactions

Authors

  • Michael Schwering,

    1. Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51-220
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  • Dr. Alexander Kiel,

    1. Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51-220
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  • Anton Kurz,

    1. Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51-220
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  • Dr. Konstantinos Lymperopoulos,

    1. Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51-220
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  • Dr. Arnd Sprödefeld,

    1. Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, (Germany)
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  • Prof. Dr. Roland Krämer,

    1. Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, (Germany)
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  • Priv.-Doz. Dr. Dirk-Peter Herten

    Corresponding author
    1. Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51-220
    • Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51-220
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  • We acknowledge financial support from the DFG (EXC 81, SFB 623) and the BMBF (ForSys, VIROQUANT), and we thank Markus Sauer (Universität Würzburg) and Steve Wolter (Universität Bielefeld) for providing software for data analysis.

Abstract

original image

Revolution in resolution: Abbe's resolution limit has been overcome in fluorescence microscopy by using light-driven processes to switch the emission of fluorophores on and off. Alternatively, chemical reactions can be used, for example the coordination of Cu2+ ions to a fluorescent probe for the stochastic switching between spectroscopic states.

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