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Keywords:

  • biological cells;
  • biomolecules;
  • isotopic labeling;
  • NMR spectroscopy;
  • proteins
Thumbnail image of graphical abstract

A direct response: Exclusively heteronuclear 13C direct-detection 2D NMR experiments are performed on 13C,15N-enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well-resolved carbonyl detection (see picture), whereas folded proteins or structured motifs do not give any detectable spectrum.