Communication

13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells†

  1. Prof. Ivano Bertini1,*,
  2. Prof. Isabella C. Felli1,
  3. Dr. Leonardo Gonnelli2,
  4. Vasantha Kumar M. V.2,
  5. Prof. Roberta Pierattelli1

Article first published online: 4 FEB 2011

DOI: 10.1002/anie.201006636

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 10, pages 2339–2341, March 1, 2011

Additional Information

How to Cite

Bertini, I., Felli, I. C., Gonnelli, L., Kumar M., V. and Pierattelli, R. (2011), 13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells. Angew. Chem. Int. Ed., 50: 2339–2341. doi: 10.1002/anie.201006636

Author Information

  1. 1

    CERM and Department of Chemistry “Ugo Schiff”, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy), Fax: (+39) 055-457-4271

  2. 2

    CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy)

*CERM and Department of Chemistry “Ugo Schiff”, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy), Fax: (+39) 055-457-4271

  1. †

    This work was supported in part by EC contracts Bio-NMR no. 261863, INSTRUCT no. 211252, FIRB-Proteomica RBRN07BMCT, and by Ente Cassa di Risparmio di Firenze.

Publication History

  1. Issue published online: 24 FEB 2011
  2. Article first published online: 4 FEB 2011
  3. Manuscript Received: 22 OCT 2010

Funded by

  • EC. Grant Numbers: 261863, 211252
  • Ente Cassa di Risparmio di Firenze

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Keywords:

  • biological cells;
  • biomolecules;
  • isotopic labeling;
  • NMR spectroscopy;
  • proteins
Thumbnail image of graphical abstract

A direct response: Exclusively heteronuclear 13C direct-detection 2D NMR experiments are performed on 13C,15N-enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well-resolved carbonyl detection (see picture), whereas folded proteins or structured motifs do not give any detectable spectrum.

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