13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells

Authors

  • Prof. Ivano Bertini,

    Corresponding author
    1. CERM and Department of Chemistry “Ugo Schiff”, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy), Fax: (+39) 055-457-4271
    • CERM and Department of Chemistry “Ugo Schiff”, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy), Fax: (+39) 055-457-4271
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  • Prof. Isabella C. Felli,

    1. CERM and Department of Chemistry “Ugo Schiff”, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy), Fax: (+39) 055-457-4271
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  • Dr. Leonardo Gonnelli,

    1. CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy)
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  • Vasantha Kumar M. V.,

    1. CERM, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy)
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  • Prof. Roberta Pierattelli

    1. CERM and Department of Chemistry “Ugo Schiff”, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence (Italy), Fax: (+39) 055-457-4271
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  • This work was supported in part by EC contracts Bio-NMR no. 261863, INSTRUCT no. 211252, FIRB-Proteomica RBRN07BMCT, and by Ente Cassa di Risparmio di Firenze.

Abstract

original image

A direct response: Exclusively heteronuclear 13C direct-detection 2D NMR experiments are performed on 13C,15N-enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well-resolved carbonyl detection (see picture), whereas folded proteins or structured motifs do not give any detectable spectrum.

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