SEIRA Spectroscopy of the Electrochemical Activation of an Immobilized [NiFe] Hydrogenase under Turnover and Non-Turnover Conditions

Authors

  • Dr. Diego Millo,

    1. Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany), Fax: (+49) 30-3142-1122
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  • Prof. Dr. Peter Hildebrandt,

    1. Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany), Fax: (+49) 30-3142-1122
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  • Dr. Maria-Eirini Pandelia,

    1. Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34–36, 45470 Mülheim an der Ruhr (Germany)
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  • Prof. Dr. Wolfgang Lubitz,

    1. Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34–36, 45470 Mülheim an der Ruhr (Germany)
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  • Dr. Ingo Zebger

    Corresponding author
    1. Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany), Fax: (+49) 30-3142-1122
    • Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin (Germany), Fax: (+49) 30-3142-1122
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  • Financial support by the DFG (P.H., IZ: Cluster of Excellence “UniCat”), EU/energy network project SOLAR-H2 (FP 7, Contract 212508, W.L., M.E.P.), the Alexander von Humboldt Foundation (D.M.), and the Max Planck Society (W.L., M.E.P.). SEIRA=Surface-enhanced infrared absorption.

Abstract

original image

Effective and unaffected: An [NiFe] hydrogenase was immobilized on Au electrodes. Surface-enhanced infrared absorption (SEIRA) spectroscopy monitored structural changes of the active site as a function of the applied potential. The redox titration of the enzyme gave a formal potential (E1/2) similar to that obtained for the enzyme in solution, demonstrating that the structure of the active site is not affected by the immobilization.

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